A quick and simple introduction to Drosophila melanogaster What is it and why bother about it?
Fly food The first step in preparing culture vials is adding food media. There are a variety of types of food available for the flies; some require cooking and others are bought already prepared and dehydrated.
The latter can be purchased from a biological supply company. This is, of course, much quicker and easier than preparing cooked media, so much so that students can fill their own vials with media. However, it must be completely rehydrated for best results, since this is the only water source for adults and larvae.
Therefore, follow the suggestions below to ensure a completely hydrated media: Add water until media appears completely moistened.
Allow the vial to sit for a few minutes, adding additional water if necessary until the media is completely hydrated.
The surface should be moist with a shiny appearance and there should be no spaces in the media.
If the media is not completed hydrated, production of vigorous cultures is compromised. Flies may be added minutes after media has been hydrated. Remember to add several grains but not more of yeast to the media surface before adding flies. Keep the media out overnight to cure, keeping the vials covered with cloth to keep wild flies from laying eggs in them.
The next day, add yeast and plugs. Refrigerate any unused media vials. Cooked media can be stored in a refrigerator for several weeks. Allow media to warm to room temperature before adding flies.
Do not allow media to dry out. Environment The easiest way to grow flies is at room temperature. In these conditions generation time is shorter days from egg to adult. Unless equipment is readily available this is unnecessary for successful rearing and crossing of flies.
It is preferable to keep flies out of drafts and direct sunlight or heat sources. These will rapidly dry the media, necessitating frequent media changes and the potential to dehydrate the flies. Anesthetizing flies The problem with fruit flies is that they fly! Therefore a variety of methods have been developed to anesthetize flies.
Include are ether, commercial brands such as Flynap, carbon dioxide, and cooling. Each has its strengths and weaknesses. Ether is flammable, has a strong odor and will kill flies if they are over-etherized and can anesthetize younger students!
Flynap, from Carolina Biological, is messy and has an odor that some find offensive.INTRODUCTION TO DROSOPHILA GENETICS DROSOPHILA CULTURE We will study basic principles of Mendelian inheritance with the use of the fruit fly, Drosophila melanogaster [the name means “black-bodied fruit-lover”].
Free Essay: INTRODUCTION TO DROSOPHILA GENETICS DROSOPHILA CULTURE We will study basic principles of Mendelian inheritance with the use of the fruit fly. Introduction to Biotechnology: An Essential Curriculum, Page 1; Building Blocks of Science.
Building Blocks of Science.
Building Blocks of Science Elementary Curriculum offers kits that are affordable and easy to implement in your classroom.
Introductory Genetics with Drosophila. Finally, applications of Drosophila research, ranging from genetics to cardiac and neurological development and disease, are provided. This video serves as an overview of the highly-important and influential model organism that is Drosophila melanogaster.
INTRODUCTION TO DROSOPHILA GENETICS DROSOPHILA CULTURE We will study basic principles of Mendelian inheritance with the use of the fruit fly, Drosophila melanogaster [the name means “black-bodied fruit-lover”].
Probably the easiest simple introduction to Drosophila development and quite readable. Drosophila (Brian Shorrocks; Ginn & Co, London, ) An interesting slim volume looking at the general biology of the fly, with chapters on laboratory and field ecology, simple genetics, behaviour and techniques, from a zoological perspective.